Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion

Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent is rich in carbon nitrogen can replace expensive feedstock used during the fermentation process. The main objective of this study was to achieve a biopesticide formulation wastewater (SIW) with high entomotoxicity (UI/ml) larvae comparable Foray 76B, which commercial biopesticide. Bacillus thuringiensis var kurstaki HD1 (Btk) strain cultivated sub-cultured aerobic, digested SIW. Pre-treatment carried on these different substrates enhance residual required growth delta endotoxin After 48 hours fermentation, cells count delta-endotoxin were determined. A containing fermented broth adjuvants fed determine mortality. cell sporulation profile SIW media displayed total viable spores compared btk or after 48h fermentation. maximum concentration medium 435μg/mL, whereas, effluent, concentrations at 161 μg/mL 136 μg/mL, respectively. When acidic treatment performed pH 2 substrates, obtained formulations significantly higher entomoxicity than untreated ones. treated equivalent standard 76B 20,000 IU/μL. Anaerobic did not enough organic augment entomotoxicity. Substrates pre-treated provided significant matter resulted larval mortality com ercial 76B.